OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-VAMP8 antibodies

Anti-VAMP8 Antibody EP2629Y

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301228
  • Rabbit Monoclonal Antibody against VAMP8 (Clone EP2629Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401238) , 20ug Explanation
100ul 325 In Stock
Add to Shopping Cart
spacer
WB(1)
IHC(2)
spacer
Also for VAMP8 (NM_003761)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human VAMP8 was used as an immunogen.
Clone NameEP2629Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1: 10,000~20,000; IHC: 1: 100~250; ICC: 1: 100~250; FC: 1:80; IP: 1:20
Predicted MW Explanation 11 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens vesicle-associated membrane protein 8 (VAMP8)
Alternative NameEDB; VAMP-8
Database LinkNP_003752
Entrez Gene 8673 Human
Entrez Gene 22320 Mouse
Entrez Gene 83730 Rat
FunctionSynaptobrevins/vesicle-associated membrane proteins (VAMPs) together with syntaxins and a synaptosome-associated protein of 25 kDa (SNAP-25) are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane (1). The terminal step of cytokinesis in animal cells is the abscission of the midbody, a cytoplasmic bridge that connects the two prospective daughter cells. It has been shown that two members of the SNARE membrane fusion machinery, syntaxin 2 and endobrevin/VAMP8, specifically localize to the midbody during cytokinesis in mammalian cells (2). It has been established that VAMP8/endobrevin is a major player in regulated exocytosis of the exocrine pancreas. VAMP8 is enriched on the membrane of zymogen granules and exists in a complex with syntaxin 4 and SNAP-23. Results suggest a major physiological role of VAMP8 in regulated exocytosis of pancreatic acinar cells by serving as a v-SNARE of zymogen granules (3).
Related PathwayTransmembraneStem cell - Pluripotency SNARE interactions in vesicular transport

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - VAMP8 antibody [EP2629Y]; All lanes : Anti-VAMP8 antibody [EP2629Y] at 1/20000 dilution.Lane 1 : 293T cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : PC12 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 11 kDa.Observed band size : 17 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - VAMP8 antibody [EP2629Y]; TA301228, at a 1/100 dilution, staining VAMP8 in paraffin embedded human brain tissue by Immunohistochemistry. An HRP/AP polymerized secondary antibody was used.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP8 antibody [EP2629Y]; Immunohistochemical analysis of Human lacrimal gland tissue, staining VAMP8 with TA301228. Antigen retrieval was performed using antigen retrieval solution in a microwave. Sections were blocked with 10 goat serum for 30 minutes and incubated with primary antibody (1/100) overnight at 4°C. Staining was detected using DAB.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title