Invalid object name 'antibody_LocusMapping'. Anti-UVRAG Antibody, - OriGene
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Anti-UVRAG Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA324649
  • Rabbit polyclonal UVRAG Antibody (C-term)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC418725) , 20ug Explanation
400ul 325 3-7 Days
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WB(1)
IHC(1)
IF(2)

OriGene Data

ImmunogenThis UVRAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 666-699 amino acids from the C-terminal region of human UVRAG.
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse Concentration2.0 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsIF: 1:10~50, WB: 1:1000, IHC: 1:50~100
BufferPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Reference Data

Target NameHomo sapiens UV radiation resistance associated (UVRAG)
Alternative NameDHTX; p63; VPS38
Database LinkNP_003360
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Immunoprecipitation and western blot analysis of anti-UVRAG Pab (Cat.#TA324649) in 293T cells. UVRAG is immunoprecipitated (Lane 2) and detected in 293T cell transiently transfected with mouse UVRAG (Lane 1). Detection of endogenous UVRAG is shown in 293T cells (Lane 3) but is reduced by UVRAG siRNA transfection (Lane 4). Data courtesy of Dr. Hong-Gang Wang, Moffitt Cancer Center, Tampa, FL.
IHC Image
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with hUVRAG (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
IF Image
Immunofluorescence staining of Autophagy UVRAG antibody (Cat# TA324649) on Methanol-fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland.
IF Image
Fluorescent image of U251 cells stained with UVRAG (C-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with TA324649 UVRAG (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). UVRAG immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

 

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