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Anti-USP7 Antibody EPR4253


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SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against USP7(clone EPR4253)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401171) , 20ug Explanation
100ul 325 3-7 Days
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human USP7 was used as an immunogen.
Clone NameEPR4253 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC/IF: 1:100 - 1:250
Predicted MW Explanation 128 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens ubiquitin specific peptidase 7 (herpes virus-associated) (USP7), transcript variant 1
Alternative NameHAUSP; TEF1
Database LinkNP_003461
Entrez Gene 7874 Human
Entrez Gene 252870 Mouse
Entrez Gene 360471 Rat
FunctionUSP7 (Ubiquitin-specific-processing protease 7) is a deubiquitinase that has been shown to regulate the p53-Mdm2 pathway (1). Cotransfection of p53 and USP7 stabilizes p53 through the removal of ubiquitin moieties from polyubiquitinated p53 (1). It also counteracts the destabilizing effect of Mdm2 by direct deubiquitination of p53 (2). Subsequently, USP7 has been shown to deubiquitinate Mdm2 and Mdmx, thereby stabilizing these proteins (2). USP7 interacts with herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110 and Epstein-Barr virus EBNA1. EBNA1 shows a tenfold higher affinity than TP53 and can compete with it for USP7 binding (3).
Related PathwayProteaseDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - HAUSP / USP7 antibody [EPR4253]; All lanes : Anti-HAUSP / USP7 antibody [EPR4253] at 1/1000 dilution.Lane 1 : Ramos cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : A431 cell lysate.Lane 4 : MCF7 cell lysate.Lane 5 : C6 cell lysate.Lane 6 : RAW 264.7 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 128 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HAUSP / USP7 antibody [EPR4253]; TA307354, at 1/50, staining HAUSP / USP7 in Human colon tissue by Immunohistochemistry, Paraffin-embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - HAUSP / USP7 antibody [EPR4253]; TA307354, at 1/100, staining HAUSP / USP7 in HeLa cells by Immunofluorescence.


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