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UltraMAB

ABAT UltraMAB Antibody, Clone UMAB180

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations Customer Reviews Product Documents
SKU Description Datasheet Price Availability*  
UM800072
  • ABAT mouse monoclonal antibody,clone UMAB180, 100 ul
$468 In Stock Add to Shopping Cart
UM870072
  • ABAT mouse monoclonal antibody,clone UMAB180, 30 ul
$120 In Stock Add to Shopping Cart
UM800072CF
  • Carrier-free (BSA/glycerol-free) ABAT mouse monoclonal antibody,clone UMAB180
$528 3 days Add to Shopping Cart
WB(2)
Data Img
IHC(4)
Data Img
IF(1)
Data Img

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 29-323 of human ABAT(NP_065737) produced in E.coli.
Clone NameClone UMAB180 IsotypeIgG1
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested Dilutions IHC 1:1000,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from cell culture supernatant by affinity chromatography

Reference Data

Target NameHomo sapiens 4-aminobutyrate aminotransferase (ABAT), transcript variant 1
Alternative NameGABA-AT; GABAT; NPD009
Database LinkNP_065737
Function 4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008].
Related Pathway

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Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500
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Western blot analysis of extracts (15ug) from 9 Human tissue by using anti-ABAT monoclonal antibody (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: colon:;9:Spleen).(1:500) Dilution: 1:500
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Immunohistochemical staining of paraffin-embedded human liver tissue using ABAT clone UMAB180, mouse monoclonal antibody. Using heat-induced epitope retrieval with 1mM EDTA in 10mM Tris buffer (pH8.0) at 110°C for 3 min prior in pressure chamber/cooker to UM800072 diluted 1:1000 application. Detection with HRP enzyme and DAB chromogen was use to visualize result. Strong cytoplasmic and membranous staining is seen in the hepatocytes. Staining was not observed in the bile ducts of the liver.
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110°C for 10 min, UM800072)(1:1000)
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Immunohistochemical staining of paraffin-embedded in human thyroid carcinoma using ABAT clone UMAB180 mouse monoclonal antibody. Heat-induced epitope retrieval with 1mM EDTA in 10mM Tris buffer (pH8.0) at 110°C for 3 min prior to UM800072 diluted 1:1000 application. Detection with HRP enzyme and DAB chromogen was use to visualize result. Image shows strong cytoplasmic and membranous staining is present in the tumor cells.
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Immunohistochemical staining of paraffin-embedded human liver using ABAT clone UMAB180, mouse monoclonal antibody at 1:1500 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM800072 requires heat-induced epitope retrieval with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The image shows strong cytoplasmic and membranous staining of the hepatocytes no staining in the bile duct.
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Immunofluorescent staining of HepG2 cells using anti-ABAT mouse monoclonal antibody (UM800072, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).
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