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VSNL1 UltraMAB Antibody, Clone Clone UMAB116
UltraMAB Antibodies - Validated with 10K Protein Chip
Also for VSNL1 (NM_003385)
|Human recombinant protein fragment corresponding to amino acids 2-191 of human VSNL1 (NP_003376) produced in E.coli.|
|Clone Clone UMAB116
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
|| IHC 1:200,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens visinin-like 1 (VSNL1)|
|HLP3; HPCAL3; HUVISL1; VILIP; VILIP-1|
|This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]. |
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Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).
Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Colon, 9: Spleen, 10: Thyroid). Diluation: 1:500.
Western blot of mouse tissue lysates (20ug) from Brian. Primary antibody diluation: 1:500. Secondary antibody dilution: Mouse TrueBlot® Ultra (1:1000).
Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-VSNL1 mouse monoclonal antibody. (UM800035; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded human pancreas using anti-VSNL1 clone UMAB116 mouse monoclonal antibody (UM800035) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using
Immunohistochemical staining of paraffin-embedded human brain using anti-VSNL1 clone UMAB116 mouse monoclonal antibody (UM800035) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pr
Immunofluorescent staining of HepG2 cells using anti-VSNL1 mouse monoclonal antibody (UM800035, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).
Confocal immunofluoresce image of primary rat neurons labeled with anti-VSNL1 mouse monoclonal antibody (UM800035, green, 1:100). Actin filaments were labeled with TRICT-Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.