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CD4 UltraMAB Antibody, Clone UMAB64
UltraMAB Antibodies - Validated with 10K Protein Chip
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|Full length human recombinant protein of human CD4 (NP_000607) produced in HEK293T cell.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
|| IHC 1:50~100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens CD4 molecule (CD4), transcript variant 1|
|This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]. |
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Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Colon, 9: Spleen, 10: Thyroid). Diluation: 1:500.
Western Blot analysis of HL-60 and SW-620 cell lysates (35µg) by using anti-CD4 monoclonal antibody (Clone UMAB64)
Immunohistochemical staining of paraffin-embedded human tonsil using anti-CD4 clone UMAB64 mouse monoclonal antibody at 1:200 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. UM800010 requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber . The image shows the germinal and non germinal cells have weak to medium cytoplasmic and memeranous staining.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD4 mouse monoclonal antibody. (UM800010; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CD4 mouse monoclonal antibody. (UM800010; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunofluorescent staining of Jurkat cells using anti-CD4 mouse monoclonal antibody (UM800010, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 4µm.