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UltraMAB

ABAT UltraMAB Antibody, Clone UMAB178

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations Customer Reviews Product Documents
SKU Description Datasheet Price Availability*  
UM800070
  • ABAT mouse monoclonal antibody,clone UMAB178, 100 ul
$468 In Stock Add to Shopping Cart
UM870070
  • ABAT mouse monoclonal antibody,clone UMAB178, 30 ul
$120 In Stock Add to Shopping Cart
UM800070CF
  • Carrier-free (BSA/glycerol-free) ABAT mouse monoclonal antibody,clone UMAB178
$528 3 days Add to Shopping Cart
WB(2)
Data Img
IHC(6)
Data Img
IF(1)
Data Img
10K-CHIP(1)
Data Img

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 29-323 of human ABAT(NP_065737) produced in E.coli.
Clone NameClone UMAB178 IsotypeIgG1
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, 10K-CHIP Suggested Dilutions IHC 1:100~200,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from cell culture supernatant by affinity chromatography

Reference Data

Target NameHomo sapiens 4-aminobutyrate aminotransferase (ABAT), transcript variant 1
Alternative NameGABA-AT; GABAT; NPD009
Database LinkNP_065737
Function4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008].
Related Pathway

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Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).
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Western blot analysis of extracts (15ug) from 9 Human tissue by using anti-ABAT monoclonal antibody (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: colon:;9:Spleen).(1:500) Dilution: 1:500
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Immunohistochemical staining of paraffin-embedded carcinoma of human kidney tissue using ABAT clone UMAB178, mouse monoclonal antibody. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800070 was diluted 1:200 using HRP detection and DAB chromogen. Images shows strong cytoplasmic and membranous staining is present in the tumor cells.
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Immunohistochemical staining of paraffin-embedded carcinoma of human liver tissue using ABAT clone UMAB178, mouse monoclonal antibody. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800070 was diluted 1:200 using HRP detection and DAB chromogen. Images shows strong cytoplasmic and membranous staining is present in the tumor cells.
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Immunohistochemical staining of paraffin-embedded human pancreas tissue using ABAT clone UMAB178, mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, UM800070was diluted 1:200 and detection shown with HRP enzyme and DAB chromogen. Images shows strong cytoplasmic and membranous staining is present in the exocrine gladular cells.
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Immunohistochemical staining of paraffin-embedded carcinoma of human pancreas tissue using ABAT clone UMAB178, mouse monoclonal antibody. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800070 was diluted 1:200 and detection shown with HRP enzyme and DAB chromogen. Images shows strong cytoplasmic and membranous staining is present in the tumor cells.
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ABAT mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3 min, UM800070)(1:200)
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Immunohistochemical staining of paraffin-embedded human liver using ABAT clone UMAB178, mouse monoclonal antibody at 1:200 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. UM800070 requires heat-induced epitope retrieval with citrate pH6.0 at 110C for 3 min using pressure chamber/cooker. The image shows strong cytoplasmic and membranous staining of the hepatocytes no staining in the bile duct.
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Immunofluorescent staining of HepG2 cells using anti-ABAT mouse monoclonal antibody (UM800070, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).
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OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ABAT mouse monoclonal antibody (UM800070). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.(1:100)
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