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Home Antibody UltraMAB

UltraMAB

RAPGEF1 UltraMAB Antibody, Clone UMAB140

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations (0) Related Products Product Documents
SKU Description Datasheet Price Availability*  
UM500102
  • RAPGEF1 mouse monoclonal antibody,clone UMAB140, 100 ul
$468 In Stock Add to Shopping Cart
UM570102
  • RAPGEF1 mouse monoclonal antibody,clone UMAB140, 30 ul
$120 In Stock Add to Shopping Cart
WB(1)
Data Img
IHC(3)
Data Img
IF(1)
Data Img
10K-CHIP(1)
Data Img

OriGene Data

ImmunogenFull length human recombinant protein of human RAPGEF1(NP_941372) produced in HEK293T cell.
Clone NameClone UMAB140 IsotypeIgG1
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, 10K-CHIP Suggested Dilutions IHC 1:100~200,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 2
Alternative NameC3G; GRF2
Database LinkNP_941372
FunctionThis gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008].
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-RAPGEF1 mouse monoclonal antibody. (UM500102, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RAPGEF1 mouse monoclonal antibody. (UM500102, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human tonsil using anti-RAPGEF1mouse monoclonal antibody. (UM500102, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
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Immunofluorescent staining of HeLa cells using anti-RAPGEF1 mouse monoclonal antibody (UM500102, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.
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OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-RAPGEF1 mouse monoclonal antibody (UM500102). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
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