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RAPGEF1 UltraMAB Antibody, Clone UMAB140
UltraMAB Antibodies - Validated with 10K Protein Chip
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Also for RAPGEF1 (NM_198679)
|Full length human recombinant protein of human RAPGEF1(NP_941372) produced in HEK293T cell.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, 10K-CHIP
|| IHC 1:100~200,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 2|
|This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]. |
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Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-RAPGEF1mouse monoclonal antibody. (UM500102, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RAPGEF1 mouse monoclonal antibody. (UM500102, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-RAPGEF1 mouse monoclonal antibody. (UM500102, heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.0, 120C for 3min)
Immunofluorescent staining of HeLa cells using anti-RAPGEF1 mouse monoclonal antibody (UM500102, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-RAPGEF1 mouse monoclonal antibody (UM500102). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.