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AFP UltraMAB Antibody, Clone UMAB66
UltraMAB Antibodies - Validated with 10K Protein Chip
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|Full length human recombinant protein of human AFP (NP_001125) produced in HEK293T cell.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, 10K-CHIP
||IHC 1:100, IF 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens alpha-fetoprotein (AFP)|
|AFPD; FETA; HPAFP|
|This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]. |
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AFP monoclonal antibody (Clone UMAB66).
Immunohistochemical staining of paraffin-embedded human placenta using anti-AFP clone UMAB66 mouse monoclonal antibody at 1:200 dilution of 0.8mg/mL and detection with Polink2 Broad HRP DAB. UM500056 requires heat-induced epitope retrieval with citrate pH6.0 at 95-100C 20 minutes. The image shows strong membranous, cytoplasmic and rare nuclear protein in the trophoblast cells. This protein is also seen as secreted protein.
Immunohistochemical staining of paraffin-embedded human embryonic liver tissue using anti-AFP mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min in pressure chamber/cooker. UM500056 diluted 1:100 and detection shown with HRP enzyme and DAB chromogen. Strong cytoplasmic and membranous staining is seen in the hepatocytes.
Immunofluorescent staining of HepG2 cells using AFP mouse monoclonal antibody (UM500056, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-AFP mouse monoclonal antibody (UM500056). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.