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UltraMAB

b-Catenin UltraMAB Antibody, Clone UMAB15

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations (0) Related Products Product Documents
SKU Description Datasheet Price Availability*  
UM500015
  • Beta-Catenin (CTNNB1) mouse monoclonal antibody, clone UMAB15, 100 ul
$468 In Stock Add to Shopping Cart
UM570015
  • CTNNB1 mouse monoclonal antibody, clone UMAB15, 30 ul
$120 In Stock Add to Shopping Cart
WB(3)
Data Img
IHC(4)
Data Img
IF(1)
Data Img
FC(1)
Data Img
10K-CHIP(1)
Data Img
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Also for b-Catenin (NM_001904)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human CTNNB1 (NP_001895) produced in HEK293T cell.
Clone NameClone UMAB15 IsotypeIgG1
Species ReactivityHuman, Mouse, Rat, Monkey Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, FC, 10K-CHIP Suggested DilutionsWB 1:500, IHC 1:100, IF 1:100, FLOW 1:100
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens catenin (cadherin-associated protein), beta 1, 88kDa (CTNNB1), transcript variant 1
Alternative Namearmadillo; CTNNB; MRD19
Database LinkNP_001895
FunctionThe protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CTNNB1 monoclonal antibody.
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Western Blot analysis of 10 different human tissue lysates (10ug) by using Anti-ß-Catenin monoclonal antibody (Clone UMAB15, 1:500)
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Western analysis of HEK293T cells without treatment or treated with 30mM LiCl for 16 hours, using anti-beta-catenin antibody (clone UMAB15). Anti-HSP90 (TA500494) was used as internal loading control.
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Immunohistochemical staining of paraffin-embedded Human normal colon and colorectal carcinoma tissues using anti-beta-catenin mouse monoclonal antibody. Note: beta-catenin is mostly located at membrane/cytoplasmic region, but exhibited intensive nuclear s
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Immunohistochemical staining of paraffin-embedded human normal lung and lung Adenocarcinoma tissues using anti-beta-catenin mouse monoclonal antibody. (Clone UMAB15, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3mi
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Immunohistochemical staining of paraffin-embedded human normal ovary and Adenocarcinoma of the ovary tissues using anti-beta-catenin mouse monoclonal antibody. (Clone UMAB15, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120
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Immunohistochemical staining of paraffin-embedded Mouse colon tissue using anti-beta-catenin mouse monoclonal antibody. (Clone UMAB15, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunofluorescent image of HT-29 cells without treatment (left) or treated with 30mM LiCl (right), using anti-beta-catenin antibody (Clone UMAB15). Nuclei were labeled by Hoechst 33242 (blue).
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Flow cytometric Analysis of Hela cells, using anti-CTNNB1 antibody(UM500015),(Red), compared to a nonspecific negative control antibody,(Blue).
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OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-beta-catenin mouse monoclonal antibody (Clone UMAB15). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-beta-catenin (Clone UMAB15) very specifically recognizes beta-catenin antigen on OriGene protein microarray chip.
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