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CD19 UltraMAB Antibody, Clone UMAB103

UltraMAB™ Antibodies - Validated with 10K Protein Chip


Specifications Citations Customer Reviews Product Documents
SKU Description Datasheet Price Availability*  
  • CD19 mouse monoclonal antibody, clone UMAB103, 100 ul
$468 In Stock Add to Shopping Cart
  • CD19 mouse monoclonal antibody,clone UMAB103, 30 ul
$120 In Stock Add to Shopping Cart
CE IVD Logo CE marked antibody of this clone also available.
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OriGene Data

ImmunogenFull length human recombinant protein of human CD19(NP_001761) produced in HEK293T cell.
Clone NameClone UMAB103 IsotypeIgG1
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, FC Suggested Dilutions IHC 1:100,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography
Note This antibody is the same clone with TA506236 which is recently upgraded to be an UltraMAB.

Reference Data

Target NameHomo sapiens CD19 molecule (CD19), transcript variant 2
Alternative NameB4; CVID3
Database LinkNP_001761
FunctionLymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008].
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-CD19 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Western blot of cell lysates (35ug) from Jurkat. Diluation: 1:500
Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid 9: Colon, 10: Spleen). Diluation: 1:500.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-CD19 mouse monoclonal antibody. (UM500071; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded human tonsil using anti-CD19 clone UMAB103 mouse monoclonal antibody at 1:200 dilution of 0.6mg/mL and detection with Polink2 Broad HRP DAB. UM500071 requires heat-induced epitope retrieval with citrate pH6.0 at 95-100C 20 minutes. . The image shows strong membranous and cytoplasmic staining in >40 % of non germinal center cells of tonsil and >90% of the germinal center cells. No staining was seen in the squamous epithelia cells.
Flow Cytometric analysis of viable B-Cell Lymphoma cell line (Raji) using anti-CD19 antibody (UM500071-red histogram), compared to isotype control antibody (anti-DDK TA180144- green histogram), and no antibody control PBS (blue histogram). (CD19 titer 1:20)
Flow Cytometric analysis of viable human peripheral blood mononuclear cells (PBMCs) using anti-human CD19 antibody (UM500071-red histogram), compared to isotype control antibody (anti-DDK TA180144 - green histogram). (CD19 titer 1:20)


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