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UltraMAB

XPF UltraMAB Antibody, Clone UMAB21

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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SKU Description Datasheet Price Availability*  
UM500021
  • XPF mouse monoclonal antibody,clone UMAB21, 100 ul
$468 In Stock Add to Shopping Cart
UM570021
  • XPF mouse monoclonal antibody,clone UMAB21, 30 ul
$120 In Stock Add to Shopping Cart

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details

WB(2)
Data Img
IHC(1)
Data Img
IF(1)
Data Img
10K-CHIP(1)
Data Img
Gene NameHomo sapiens excision repair cross-complementation group 4 (ERCC4)
SynonymsERCC11; FANCQ; RAD1; XPF
ImmunogenFull length human recombinant protein of human XPF (NP_005227) produced in HEK293 cell.
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone NameClone UMAB21 IsotypeIgG1
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration0.5~1.0 mg/ml (Lot Dependent)
Purification Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *WB, IHC, IF, 10K-CHIP Suggested DilutionsWB 1:200~500, IF 1:100, FLOW 1:100, IHC 1:500
BackgroundThe protein encoded by this gene forms a complex with ERCC1 and is involved in the 5' incision made during nucleotide excision repair. This complex is a structure specific DNA repair endonuclease that interacts with EME1. Defects in this gene are a cause of xeroderma pigmentosum complementation group F (XP-F), or xeroderma pigmentosum VI (XP6).

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of crude cell extracts (35ug) from 9 different cell lines by using anti-XPF (UMAB21) monoclonal antibody.
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Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-XPF monoclonal antibody (clone UMAB21, 1:500)
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Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-XPF (UMAB21) mouse monoclonal antibody. (UM500021, Dilution 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Anti-XPF (UMAB21) mouse monoclonal antibody (UM500021) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY XPF(RC223300).
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OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-XPF mouse monoclonal antibody (clone UMAB21). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-XPF (UMAB21) very specifically recognizes XPF antigen on OriGene protein microarray chip.
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