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SERPINB4 UltraMAB Antibody, Clone UMAB16
UltraMAB Antibodies - Validated with 10K Protein Chip
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Also for SERPINB4 (NM_002974)
|Full length human recombinant protein of human SERPINB4 (NP_002965) produced in HEK293T cell. |
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, 10K-CHIP
||WB 1:500, IHC 1:150, IF 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens serpin peptidase inhibitor, clade B (ovalbumin), member 4 (SERPINB4), transcript variant 1|
|LEUPIN; PI11; SCCA-2; SCCA1; SCCA2|
|SERPINB4 is also called squamous cell carcinoma antigen 2 (SCCA-2) or leupin. It directly inhibits human granzyme proteolytic activity and that overexpression of SERPINB4 in HeLa cells inhibits recombinant granzyme-induced as well as NK cell-mediated cell death.|
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SERPINB4 monoclonal antibody (Clone UMAB16) at 1:500.
Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:1000.
Immunohistochemical staining of paraffin-embedded Carcinoma of lung tissue using anti-SERPINB4mouse monoclonal antibody. (Clone UMAB16, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-SERPINB4mouse monoclonal antibody. (Clone UMAB16, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunofluorescent staining of HepG2 cells using SERPINB4 mouse monoclonal antibody (UM500016, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-SERPINB4 mouse monoclonal antibody (Clone UMAB16). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-SERPINB4 (Clone UMAB16) very specifically recognizes SERPINB4 antigen on OriGene protein microarray chip.