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CD5 UltraMAB Antibody, Clone UMAB9
UltraMAB Antibodies - Validated with 10K Protein Chip
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|Full length human recombinant protein of human CD5 (NP_055022) produced in HEK293T cell.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB 1:500, IHC 1:100, IF 1:100, FLOW 1:100, IF 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens CD5 molecule (CD5)|
|CD5 is a cluster of differentiation found on a subset of IgM-secreting B cells called B-1 cells, and also on T cells. B-1 cells have limited diversity of theirB-cell receptor due to their lack of the enzyme terminal deoxynucleotidyl transferase (TdT) and are potentially self-reactive. CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli (such as bacterial proteins) and not by normal tissue proteins. CD5 is a good immunohistochemical marker for T-cells. About 76% of T-cell neoplasms are reported to express CD5, and it is also found in chronic lymphocytic leukemia, hairy cell leukaemia, and mantle cell lymphoma cells. It is commonly lost in cutaneous T-cell lymphoma, and its absence can be used as an indicator of malignancy in this condition. The absence of CD5 in T cell acute lymphoblastic leukaemia, while relatively rare, is associated with a poor prognosis.|
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Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-CD5 monoclonal antibody (clone UMAB9, 1:500)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD5 monoclonal antibody (Clone UMAB9) at 1:500.
Immunohistochemical staining of paraffin-embedded human tonsil using anti-CD5 clone UMAB9 mouse monoclonal antibody at 1:200 dilution of 0.1mg/mL detection with Polink2 Broad HRP DAB (UM500009 requires heat-induced epitope retrieval with Citrate pH6.0 at 95-100C 20 minutes. The image shows strong membranous and cytoplasmic staining in >75 % of non germinal center cells of tonsil and <20% of the germinal center cells. No staining was seen in the squamous epithelia cells.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD5 mouse monoclonal antibody. (Clone UMAB9, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunofluorescent staining of Jurkat cells using CD5 mouse monoclonal antibody (UM500009, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(UM500009),(Red), compared to a nonspecific negative control antibody,(Blue).