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Home Antibody UltraMAB

UltraMAB

CD5 UltraMAB Antibody, Clone UMAB9

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations Customer Reviews Product Documents
SKU Description Datasheet Price Availability*  
UM500009
  • CD5 mouse monoclonal antibody, clone UMAB9, 100 ul
$468 In Stock Add to Shopping Cart
UM570009
  • CD5 mouse monoclonal antibody, clone UMAB9, 30 ul
$120 In Stock Add to Shopping Cart
UM500009CF
  • Carrier-free (BSA/glycerol-free) CD5 mouse monoclonal antibody, clone UMAB9
$528 3 days Add to Shopping Cart
WB(2)
Data Img
IHC(8)
Data Img
IF(1)
Data Img
FC(1)
Data Img

OriGene Data

ImmunogenFull length human recombinant protein of human CD5 (NP_055022) produced in HEK293T cell.
Clone NameClone UMAB9 IsotypeIgG1
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:500, IHC 1:100, IF 1:100, FLOW 1:100, IF 1:100
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from cell culture supernatant by affinity chromatography

Reference Data

Target NameHomo sapiens CD5 molecule (CD5)
Alternative NameLEU1; T1
Database LinkNP_055022
FunctionCD5 is a cluster of differentiation found on a subset of IgM-secreting B cells called B-1 cells, and also on T cells. B-1 cells have limited diversity of theirB-cell receptor due to their lack of the enzyme terminal deoxynucleotidyl transferase (TdT) and are potentially self-reactive. CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli (such as bacterial proteins) and not by normal tissue proteins. CD5 is a good immunohistochemical marker for T-cells. About 76% of T-cell neoplasms are reported to express CD5, and it is also found in chronic lymphocytic leukemia, hairy cell leukaemia, and mantle cell lymphoma cells. It is commonly lost in cutaneous T-cell lymphoma, and its absence can be used as an indicator of malignancy in this condition. The absence of CD5 in T cell acute lymphoblastic leukaemia, while relatively rare, is associated with a poor prognosis.
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD5 monoclonal antibody (Clone UMAB9) at 1:500.
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Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-CD5 monoclonal antibody (clone UMAB9, 1:500)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD5 mouse monoclonal antibody. (Clone UMAB9, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded human tonsil using anti-CD5 clone UMAB9 mouse monoclonal antibody at 1:200 dilution of 0.1mg/mL detection with Polink2 Broad HRP DAB (UM500009 requires heat-induced epitope retrieval with Citrate pH6.0 at 95-100C 20 minutes. The image shows strong membranous and cytoplasmic staining in >75 % of non germinal center cells of tonsil and <20% of the germinal center cells. No staining was seen in the squamous epithelia cells.
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Immunohistochemical staining of paraffin-embedded mouse ascending colon tissue using anti-CD5 clone UMAB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, UM500009)(1:300).
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Immunohistochemical staining of paraffin-embedded mouse ascending colon tissue within the normal limits using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA501376)
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Immunohistochemical staining of paraffin-embedded mouse thymus tissue within the normal limits using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA501376)
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Immunohistochemical staining of paraffin-embedded mouse thymus tissue using anti-CD5 clone UMAB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, UM500009)(1:300).
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Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-CD5 clone UMAB9 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, UM500009)(1:300).
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Immunohistochemical staining of paraffin-embedded mouse spleen tissue within the normal limits using anti-CD5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, UM500009)(1:300)
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Immunofluorescent staining of Jurkat cells using CD5 mouse monoclonal antibody (UM500009, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
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Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(UM500009),(Red), compared to a nonspecific negative control antibody,(Blue).
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