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CD5 UltraMAB Antibody, Clone UMAB9
UltraMAB Antibodies - Validated with 10K Protein Chip
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|Full length human recombinant protein of human CD5 (NP_055022) produced in HEK293T cell.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB 1:500, IHC 1:100, IF 1:100, FLOW 1:100, IF 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens CD5 molecule (CD5)|
|CD5 is a cluster of differentiation found on a subset of IgM-secreting B cells called B-1 cells, and also on T cells. B-1 cells have limited diversity of theirB-cell receptor due to their lack of the enzyme terminal deoxynucleotidyl transferase (TdT) and are potentially self-reactive. CD5 serves to mitigate activating signals from the BCR so that the B-1 cells can only be activated by very strong stimuli (such as bacterial proteins) and not by normal tissue proteins. CD5 is a good immunohistochemical marker for T-cells. About 76% of T-cell neoplasms are reported to express CD5, and it is also found in chronic lymphocytic leukemia, hairy cell leukaemia, and mantle cell lymphoma cells. It is commonly lost in cutaneous T-cell lymphoma, and its absence can be used as an indicator of malignancy in this condition. The absence of CD5 in T cell acute lymphoblastic leukaemia, while relatively rare, is associated with a poor prognosis.|
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD5 monoclonal antibody (Clone UMAB9) at 1:500.
Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-CD5 monoclonal antibody (clone UMAB9, 1:500)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD5 mouse monoclonal antibody. (Clone UMAB9, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemical staining of paraffin-embedded human tonsil using anti-CD5 clone UMAB9 mouse monoclonal antibody at 1:200 dilution of 0.1mg/mL detection with Polink2 Broad HRP DAB (UM500009 requires heat-induced epitope retrieval with Citrate pH6.0 at 95-100C 20 minutes. The image shows strong membranous and cytoplasmic staining in >75 % of non germinal center cells of tonsil and <20% of the germinal center cells. No staining was seen in the squamous epithelia cells.
Immunofluorescent staining of Jurkat cells using CD5 mouse monoclonal antibody (UM500009, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody(UM500009),(Red), compared to a nonspecific negative control antibody,(Blue).