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Anti-UIMC1 Antibody EPR5315
Also for UIMC1 (NM_001199297)
|A synthetic peptide corresponding to residues in human RAP80 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:10000 - 1:50000; IHC-P: 1:250 - 1:500; ICC/IF: 1:50 - 1:100
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens ubiquitin interaction motif containing 1 (UIMC1), transcript variant 1|
|RAP80 is an ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. It plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1/BARD1 complex to sites of DNA damage at double-strand breaks (1). RAP80 contains an ubiquitin-interaction motif (UIM) that is associated with the complex through its interaction with CCDC98. Furthermore, it is reported to interact with the estrogen receptor a (ERa) in an agonist-dependent manner and is able to regulate ERa transcriptional activity (2).|
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Western blot - Anti-RAP80 antibody [EPR5315]; All lanes : Anti-RAP80 antibody [EPR5315] at 1/10000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : Human ovary cancer lysate.Lane 3 : 293T cell lysate.Lane 4 : HT-1080 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 80 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAP80 antibody [EPR5315]; ab124763, at 1/250, staining RAP80 in paraffin-embedded Human testis tissue by Immunohistochemistry.