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Anti-UCP3 Antibody EPR6427
Also for UCP3 (NM_003356)
|A synthetic peptide corresponding to residues in human UCP3 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, FC
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; FC: 1:10 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC/IF or IHC-P.
|Homo sapiens uncoupling protein 3 (mitochondrial, proton carrier) (UCP3), transcript variant long|
|Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. The different UCP's have tissue-specific expression; UCP3 is primarily expressed in skeletal muscle. It is postulated to protect mitochondria against lipid-induced oxidative stress. Expression levels of UCP3 increase when fatty acid supplies to mitochondria exceed their oxidation capacity, and the protein enables the export of fatty acids from mitochondria. UCP's contain the three solcar protein domains typically found in MACPs (1).|
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Western blot - Anti-UCP3 antibody [EPR6427]; All lanes : Anti-UCP3 antibody [EPR6427] at 1/1000 dilution.Lane 1 : Human testis lysate .Lane 2 : A673 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 34 kDa.
Other-Anti-UCP3 antibody [EPR6427](ab124925); Equilibrium disassociation constant (KD)..
Flow Cytometry - Anti-UCP3 antibody [EPR6427]; ab124925, at 1/10 dilution, staining UCP3 in permeabilized A673 cells by Flow cytometry.(red). .A rabbit IgG was used as negative control (green).