OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-UCHL1 antibodies

Anti-UCHL1 Antibody

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA321632
  • Anti-UCHL1 Rabbit Polyclonal Antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401345) , 20ug
100ul 325 2-3 Weeks
Add to Shopping Cart
WB(1)
spacer
Also for UCHL1 (NM_004181)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFusion protein corresponding to C terminal 165 amino acids of human ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1.03mg/ml
Guaranteed Application *WB Suggested DilutionsWB: 1:500-2000
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) (UCHL1)
Alternative NameHEL-117; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; Uch-L1
Database LinkNP_004172
FunctionProtein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes (UBEs) and deubiquitinating enzymes (DUBs). DUBs are categorized into 5 subfamilies: USP, UCH, OTU, MJD, and JAMM. UCHL1, UCHL3, UCHL5/UCH37, and BRCA-1-associated protein-1 (BAP1) belong to the UCH family of DUBs, which all posses a conserved catalytic domain (UCH domain) of about 230 amino acids. UCHL5 and BAP1 have unique extended C-terminal tails. UCHL1 is abundantly expressed in neuronal tissues and testes, while UCHL3 expression is more widely distributed. Although UCHL1 and UCHL3 are the most closely related UCH family members with about 53% identity, their biochemical properties differ in that UCHL1 binds monoubiquitin and UCHL3 shows dual specificity toward both ubiquitin (Ub) and NEDD8, a Ub-like molecule. In particular, UCHL3 functions as a Ub hydrolase involved in the processing of both Ub precursors and ubiquitinated substrates, generating free monomeric Ub. This is accomplished through the ability of UCHL3 to recognize and hydrolyze isopeptide bonds at the C-terminal glycine of either Ub or NEDD8. Recent functional studies have identified UCH-L3 as a critical regulator of adipogenesis through its ability to promote IGF-IR and insulin receptor signaling. Furthermore, UCHL3 has been shown to promote deubiquitination, recycling, and cell surface expression of the epithelial sodium channel.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 25 kDa . Positive control: A549 and Y79 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: A549 cell lysate Lane2: Y79 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title