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Anti-UCHL1 Antibody EPR4117
Also for UCHL1 (NM_004181)
|A synthetic peptide corresponding to residues in human PGP9.5 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IHC
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:50 - 1:100; ICC: 1:50 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) (UCHL1)|
|NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; Uch-L1|
|PGP9.5 belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. It is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in PGP9.5 may be associated with Parkinson disease and Alzheimer's disease.|
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Western blot - PGP9.5 antibody [EPR4117]; All lanes : Anti-PGP9.5 antibody [EPR4117] at 1/1000 dilution.Lane 1 : Human fetal brain lysate.Lane 2 : Y79 lysate.Lane 3 : U-87 MG lysate.Lane 4 : SH SY5Y lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 24 kDa.
Western blot - Anti-PGP9.5 antibody [EPR4117]; All lanes : Anti-PGP9.5 antibody [EPR4117] at 1/1000 dilution.Lane 1 : Whole tissue lysate prepared from adult mouse brain homogenate (CD-1 strain).Lane 2 : Whole tissue lysate prepared from non transfected control cells (low endogenous expression of UCH-L1).Lane 3 : Whole tissue lysate prepared from cells transfected with UCH-L1-Flag cDNA (positive control) .Lysates/proteins at 100 µg per lane.Secondary.HRP conjugated goat anti-rabiit polyclonal at 1/10000 dilution.developed using the ECL technique.Predicted band size : 24 kDa.Exposure time : 2 minutesImage courtesy of Dr Simone Diestel by Abreview.
Other-Anti-PGP9.5 antibody [EPR4117](ab109261); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PGP9.5 antibody [EPR4117]; ab109261 at 1/50 dilution staining PGP9.5 in Human brain by Immunohistochemistry, Paraffin-embedded tissue.