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Anti-UBE2M Antibody EPR5333
Also for UBE2M (NM_003969)
|A synthetic peptide corresponding to residues on the C-terminus in human UBC12 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:500 - 1:1000; FC: 1:10 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens ubiquitin-conjugating enzyme E2M (UBE2M)|
|hUbc12; UBC-RS2; UBC12|
|The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBC12 is a member of the E2 ubiquitin-conjugating enzyme family. It is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin (1).|
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Western blot - UBE2M antibody [EPR5333]; All lanes : Anti-UBE2M antibody [EPR5333] at 1/10000 dilution.Lane 1 : 293T cell lysate.Lane 2 : Ramos cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 21 kDa.Observed band size : 20 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBE2M antibody [EPR5333]; Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue using ab109507 at a dilution of 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBE2M antibody [EPR5333]; Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue using ab109507 at a dilution of 1/100.