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Anti-UBE2L3 Antibody EPR4368
Also for UBE2L3 (NM_003347)
|A synthetic peptide corresponding to residues in human UBE2L3 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||ICC/IF: 1:100 - 1:250; WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens ubiquitin-conjugating enzyme E2L 3 (UBE2L3), transcript variant 1|
|E2-F1; L-UBC; UBCH7; UbcM4|
|The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2L3 / UBCH7 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro.|
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Western blot - Ube2L3 antibody [EPR4368]; All lanes : Anti-Ube2L3 antibody [EPR4368] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : LNCaP cell lysate.Lane 3 : Jurkat cell lysate.Lane 4 : K562 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 18 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ube2L3 antibody [EPR4368]; ab108936, at a 1/100 dilution, staining Ube2L3 in paraffin-embedded Human testis tissue
Immunocytochemistry/ Immunofluorescence - Ube2L3 antibody [EPR4368]; ab108936, at a 1/100 dilution, staining Ube2L3 in HeLa cells