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Home Antibody All anti-UBD antibodies

Anti-UBD Antibody EPR4370

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307160
  • Rabbit monoclonal antibody against FAT10(clone EPR4370)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401929) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(1)
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Also for UBD (NM_006398)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human FAT10 was used as an immunogen.
Clone NameEPR4370 IsotypeTissue culture supernatant
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC: 1:100 - 1:250
BufferDoes not react with Rat. Is unsuitable for Flow Cyt or IP.
Purification pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens ubiquitin D (UBD)
Alternative NameFAT10; GABBR1; UBD-3
Database LinkNP_006389
FunctionFAT10, also known as ubiquitin D, is a ubiquitin-like protein modifier that can be covalently attached to the target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1L-dependent manner. It probably functions as a survival factor. FAT10 regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity, NF-kappa-B, by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. It is required for TNF-alpha-induced p65 nuclear translocation in renal tubular epithelial cells (RTECs). FAT10 also mediates mitotic non-disjunction and chromosome instability, in long-term in vitro culture and cancers, by abbreviating mitotic phase and impairing the kinetochore localization of MAD2L1 during the prometaphase stage of the cell cycle (1).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Diubiquitin antibody [EPR4370]; All lanes : Anti-Di-ubiquitin antibody [EPR4370] at 1/1000 dilution.Lane 1 : HepG2 cell lysate.Lane 2 : MOLT4 cell lysate.Lane 3 : K562 cell lysate.Lane 4 : Daudi cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled Goat anti-rabbit at 1/2000 dilution.Predicted band size : 18 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Diubiquitin antibody [EPR4370]; Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Diubiquitin with TA307160 at a dilution of 1/50.

 

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