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Anti-TUBA1B Antibody EPR1333
Also for TUBA1B (NM_006082)
|A synthetic peptide corresponding to residues in human Alpha-Tubulin was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:10000 - 1:50000; IHC-P: 1:500 - 1:1000; FC: 1:10 - 1:100; ICC/IF: 1:250 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens tubulin, alpha 1b (TUBA1B)|
|Microtubules are essential to eukaryotic cytoskeletal structures, vesicular transportation, and mitosis. It consists mainly of two soluble protein subunits, alpha- and beta-tubulin. Alpha-tubulin binds to beta-tubulin to form a heterodimer that is subject to post-translation modifications (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta-tubulin, but not alpha-tubulin. The stability of the dimer in the microtubules is dependent on the presence of beta-tubulin, since the dimer with GTP bound beta-tubulin is stable in microtubule integration (2). For both types of tubulins, the carboxy-terminal tail is post-translationally modified to regulate associated protein binding at the microtubule surface (3). Defects in alpha-tubulin are the cause of lissencephaly type 3 (LIS3), characterized by agyria (4).|
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Western blot - TUBA1B antibody [EPR1333]; All lanes : Anti-TUBA1B antibody [EPR1333] at 1/10000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : 293T cell lysate.Lane 4 : A431 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 50 kDa.Observed band size : 50 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TUBA1B antibody [EPR1333]; ab108629, at 1/500 dilution, staining TUBA1B in paraffin embedded Human placenta tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TUBA1B antibody [EPR1333]; ab108629, at a 1/500 dilution, staining TUBA1B in paraffin embedded Human breast tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - TUBA1B antibody [EPR1333]; ab108629, at a 1/250 dilution, staining TUBA1B in HeLa cells by Immunofluorescence.