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Home Antibody All anti-TUBA1B antibodies

Anti-TUBA1B Antibody EPR1333


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA307175 Rabbit monoclonal antibody against Alpha-Tubulin(clone EPR1333) 100ul $325 In Stock
LC401831 TUBA1B HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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Also for TUBA1B (NM_006082)
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Alpha-Tubulin was used as an immunogen.
Clone NameEPR1333 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:10000 - 1:50000; IHC-P: 1:500 - 1:1000; FC: 1:10 - 1:100; ICC/IF: 1:250 - 1:500
Predicted MW Explanation 50 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens tubulin, alpha 1b (TUBA1B)
Alternative NameK-ALPHA-1
Database LinkNP_006073
Entrez Gene 10376 Human
Entrez Gene 22143 Mouse
Entrez Gene 500929 Rat
FunctionMicrotubules are essential to eukaryotic cytoskeletal structures, vesicular transportation, and mitosis. It consists mainly of two soluble protein subunits, alpha- and beta-tubulin. Alpha-tubulin binds to beta-tubulin to form a heterodimer that is subject to post-translation modifications (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta-tubulin, but not alpha-tubulin. The stability of the dimer in the microtubules is dependent on the presence of beta-tubulin, since the dimer with GTP bound beta-tubulin is stable in microtubule integration (2). For both types of tubulins, the carboxy-terminal tail is post-translationally modified to regulate associated protein binding at the microtubule surface (3). Defects in alpha-tubulin are the cause of lissencephaly type 3 (LIS3), characterized by agyria (4).
Related PathwayDruggable Genome Gap junctionPathogenic Escherichia coli infection

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - TUBA1B antibody [EPR1333]; All lanes : Anti-TUBA1B antibody [EPR1333] at 1/10000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : 293T cell lysate.Lane 4 : A431 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 50 kDa.Observed band size : 50 kDa.

IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TUBA1B antibody [EPR1333]; TA307175, at 1/500 dilution, staining TUBA1B in paraffin embedded Human placenta tissue by Immunohistochemistry.

IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TUBA1B antibody [EPR1333]; TA307175, at a 1/500 dilution, staining TUBA1B in paraffin embedded Human breast tissue by Immunohistochemistry.

IF Image
Immunocytochemistry/ Immunofluorescence - TUBA1B antibody [EPR1333]; TA307175, at a 1/250 dilution, staining TUBA1B in HeLa cells by Immunofluorescence.


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