TRIP12 Rabbit Polyclonal Antibody

CAT#: TA349096

Rabbit Polyclonal Anti-TRIP12 Antibody

Product Images

Western blot analysis of TRIP12 in rat colon tissue lysate with TRIP12 antibody at 1 ug/mL.

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  Immunohistochemistry of TRIP12 in rat colon tissue with TRIP12 antibody at 5 ug/mL.

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  Immunofluorescence of TRIP12 in rat colon tissue with TRIP12 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, IHC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: TRIP12 antibody was raised against a 18 amino acid peptide near the carboxy terminus of human TRIP12.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: TRIP12 antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 219 kDa; Observed: 220 kDa
• Gene Name: thyroid hormone receptor interactor 12
• Database Link:
  NP_004229
  Entrez Gene 9320 Human
  UniProt ID Q14669
• Background: Thyroid hormone receptors (TRs) are transcription factors that regulate the expression of specific genes in a hormone-dependent manner (1). TRIP12 (thyroid hormone receptor interactor 12) is an ATP-dependent E3 ubiquitin ligase involved in the human ubiquitin fusion degradation (UFD) pathway and also modulates the NEDD8 pathway (2,3). TRIP12 contains one WWE domain and a single HECT (E6AP-type E3 ubiquitin-protein ligase) domain suggested to contain a noncovalent ubiquitin-binding site (4). TRIP12 acts as a key regulator of DNA damage response and the ubiquitin ligase activity of TRIP12 is essential for mouse development (5).
• Synonyms: AGR16; EDG-5; EDG5; Gpcr13; H218; LPB2; S1P2