Homo sapiens tripartite motif containing 3 (TRIM3), transcript variant 2
Synonyms:
BERP; HAC1; RNF22; RNF97
Immunogen
Peptide with sequence AKREDSPGPEVQP-C, from the N Terminus of the protein sequence according to NP_006449.2; NP_150594.2.
Buffer
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Clone Name
Isotype
Goat IgG
Species Reactivity
Human, Mouse, Rat, Dog
Concentration
0.5 mg/ml
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. (Protein A or G Sepharose)
Guaranteed Application *
WB
Suggested Dilutions
ELISA: 1:64,000. WB: 1-3µg/ml.
Background
The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin V-mediated cargo transport. Alternatively spliced transcript variants encoding the same isoform have been identified. [provided by RefSeq].
Related Pathway
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HEK293T cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY TRIM3 (RC211928) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM3.
TA302432 staining (1ug/ml) of Mouse Brain (RIPA buffer, 35ug total\nprotein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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