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Home Antibody All anti-TP53 antibodies

Anti-TP53 TRUEMAB Antibody Clone OTI2H4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


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SKU Description Amount Price Availability*  
TA502925 TP53 mouse monoclonal antibody, clone OTI2H4 (formerly 2H4) 100ul 325
In Stock
LC400186 TP53 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502925 Carrier-free (BSA/glycerol-free) TP53 mouse monoclonal antibody, clone OTI2H4 (formerly 2H4) 100ug $450 3-4 weeks
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OriGene Data

ImmunogenFull length human recombinant protein of human TP53 (NP_000537) produced in HEK293T cell.
Clone NameClone OTI2H4 IsotypeIgG1
Species ReactivityHuman, Monkey Concentration0.69 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:500~2000, IF 1:100, FLOW 1:100
Predicted MW Explanation 43.5 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens tumor protein p53 (TP53), transcript variant 1
Alternative NameBCC7; LFS1; P53; TRP53
Database LinkNP_000537
Entrez Gene 7157 Human
Entrez Gene 716170 Monkey
FunctionThe protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq].
Related PathwayStem cell - PluripotencyTranscription FactorsDruggable Genome MAPK signaling pathwayCell cyclep53 signaling pathwayApoptosisWnt signaling pathwayNeurotrophin signaling pathwayMore Pathways >>

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* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53 (RC200003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TP53.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TP53 monoclonal antibody.
Anti-TP53 mouse monoclonal antibody (TA502925) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TP53(RC200003).
HEK293T cells transfected with either RC200003 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TP53 antibody(TA502925), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-TP53 antibody(TA502925),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-TP53 antibody(TA502925),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).


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