Anti-TP53 Antibody, clone 2H4

Also for TP53 (NM_000546)

OriGene Data

Immunogen	Full length human recombinant protein of human TP53 (NP_000537) produced in HEK293T cell.
Clone Name	clone 2H4	Isotype	IgG1
Species Reactivity	Human, Monkey	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IF, FC	Suggested Dilutions	WB 1:500~2000, IF 1:100, FLOW 1:100
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens tumor protein p53 (TP53), transcript variant 1
Alternative Name	BCC7; LFS1; P53; TRP53
Database Link	NP_000537
Function	The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq].
Related Pathway	Apoptosis Cell cycle Delta-Notch Signaling Pathway MAPK signaling pathway Senescence and Autophagy TGF Beta Signaling Pathway Wnt Signaling Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53 (RC200003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TP53.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TP53 monoclonal antibody.

IF Image

Anti-TP53 mouse monoclonal antibody (TA502925) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TP53(RC200003).

FC Image

HEK293T cells transfected with either RC200003 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TP53 antibody(TA502925), and then analyzed by flow cytometry.

FC Image

Flow cytometric Analysis of Hela cells, using anti-TP53 antibody(TA502925),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-TP53 antibody(TA502925),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

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Anti-TP53 TrueMAB Antibody clone 2H4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data