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Anti-TP53 TRUEMAB Antibody clone 1D8
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for TP53 (NM_000546)
|Full length human recombinant protein of human TP53 (NP_000537) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens tumor protein p53 (TP53), transcript variant 1|
|BCC7; LFS1; P53; TRP53|
Entrez Gene 7157 Human
Entrez Gene 716170 Monkey
|The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq]. |
|Stem cell - PluripotencyTranscription FactorsDruggable Genome MAPK signaling pathwayCell cyclep53 signaling pathwayApoptosisWnt signaling pathwayNeurotrophin signaling pathwayMore Pathways >> |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53 (RC200003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TP53.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TP53 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502851)
Anti-TP53 mouse monoclonal antibody (TA502851) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TP53(RC200003).
HEK293T cells transfected with either RC200003 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TP53 antibody(TA502851), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-TP53 antibody(TA502851),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-TP53 antibody(TA502851),(Red), compared to a nonspecific negative control antibody,(Blue).