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Home Antibody All anti-TOP2A antibodies

Anti-TOP2A PHOSPHO Antibody EP1054Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303722
  • Rabbit monoclonal antibody against Topoisomerase II alpha Phospho (pT1343)(EP1054Y) (phospho-specific)
100ul $325 3-7 Days
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WB(1)
IHC(1)

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Thr 1343 of human Topo II alpha was used as immunogen. The antibody only detects Topo II alpha phosphorylated on Threonine 1343.
Clone NameEP1054Y IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC Suggested DilutionsIHC-P: Use at an assay dependent dilution; WB: 1:1000; ICC: 1:50 - 1:100
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens topoisomerase (DNA) II alpha 170kDa (TOP2A)
Alternative NameTOP2; TP2A
Database LinkNP_001058
FunctionThis gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq].
Related Pathway

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WB Image
Western blot - Topoisomerase II alpha (phospho T1343) antibody [EP1054Y]; All lanes : Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] at 1/1000 dilution.Lane 1 : HeLa cell lysate treated with AP.Lane 2 : HeLa cell lysate untreated.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 174 kDa.Observed band size : 174 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Topoisomerase II alpha (phospho T1343) antibody [EP1054Y]; TA303722 at 1/100 dilution staining Topoisomerase II alpha in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.

 

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