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Anti-TOP1 Antibody EPR5375
Also for TOP1 (NM_003286)
|A synthetic peptide corresponding to residues on the N-terminus in human Topoisomerase 1 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:10000 - 1:50000; IHC-P: 1:100 - 1:250; FC: 1:10 - 1:100; ICC/IF: 1:100 - 1:250
|Does not react with Rat. Is unsuitable for IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens topoisomerase (DNA) I (TOP1)|
|Topoisomerase 1 is an enzyme that controls and alters the topologic states of DNA during transcription. It catalyzes the transient breaking and rejoining of a single strand of DNA, which allows the strands to pass through one another, thus altering the topology of DNA (1). Topoisomerase 1 has been further classified into two different families, types IA and IB, depending on whether they form a transient covalent bond with the 5′ or 3′ end of the broken DNA strand (2). Eukaryotic topoisomerase 1 and 2 can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils (3).|
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Western blot - Topoisomerase I antibody [EPR5375]; All lanes : Anti-Topoisomerase I antibody [EPR5375] at 1/10000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : MCF7 cell lysate.Lane 3 : SW480 cell lysate.Lane 4 : K562 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 91 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Topoisomerase I antibody [EPR5375]; Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using ab109374 at a dilution of 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Topoisomerase I antibody [EPR5375]; Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue using ab109374 at a dilution of 1/100.
Immunocytochemistry/ Immunofluorescence - Topoisomerase I antibody [EPR5375]; Immunofluorescent staining of MCF7 cells using ab109374 at a dilution of 1/100.
Flow Cytometry - Anti-Topoisomerase I antibody [EPR5375]; Overlay histogram showing HepG2 cells stained with ab109374 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.