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Anti-TNF Antibody Polyclonal
|E.coli derived Recombinant Human TNF-Î± |
||Lot dependent; please refer to CoA along with shipment
|A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.|
|Produced from sera of goats pre-immunized with highly pure recombinant Human TNF-alpha. Anti-Human TNF-alpha specific antibody was purified by affinity chromatography employing immobilized Human TNF-alpha matrix.
|Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human TNF-alpha (0.25 ng/ml), a concentration of 0.012 - 0.014 <=ug/ml of this antibody is required.
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To detect Human TNF-alpha; by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human TNF-alpha; (60-325GBT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TNF-alpha;.
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 2.5 ug/ml-5.0 ug/ml with a two-hour incubation at room temperature or an overnight incubation at 4C. An HRP-labeled polymer detection system was used with a DAB chromogen. This antibody was also tested in an HRP-biotin labeling detection system. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.