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Anti-TNF Antibody Polyclonal

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA328454
  • Anti-Human TNF-a Goat Polyclonal Antibody
100ug 325 3-7 Days
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IHC(1)
ELISA(1)
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Also for TNF (NM_000594)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenE.coli derived Recombinant Human TNF-α
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *ELISA, IHC Suggested DilutionsWB,ELISA,IHC
BufferA sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Purification Produced from sera of goats pre-immunized with highly pure recombinant Human TNF-alpha. Anti-Human TNF-alpha specific antibody was purified by affinity chromatography employing immobilized Human TNF-alpha matrix.
Note Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human TNF-alpha (0.25 ng/ml), a concentration of 0.012 - 0.014 <=ug/ml of this antibody is required.

Reference Data

Target NameHomo sapiens tumor necrosis factor (TNF)
Alternative NameDIF; TNF-alpha; TNFA; TNFSF2
Database LinkNP_000585
Entrez Gene 7124 Human
FunctionCentrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Related PathwaySecreted ProteinTransmembraneTranscription FactorsDruggable Genome MAPK signaling pathwayCytokine-cytokine receptor interactionApoptosisTGF-beta signaling pathwayToll-like receptor signaling pathwayNOD-like receptor signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

ELISA Image
To detect Human TNF-alpha; by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human TNF-alpha; (60-325GBT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TNF-alpha;.
IHC Image
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 2.5 ug/ml-5.0 ug/ml with a two-hour incubation at room temperature or an overnight incubation at 4C. An HRP-labeled polymer detection system was used with a DAB chromogen. This antibody was also tested in an HRP-biotin labeling detection system. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.

 

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