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Home Antibody All anti-TMPRSS2 antibodies

Anti-TMPRSS2 Antibody EPR3861

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307198
  • Rabbit monoclonal antibody against TMPRSS2 (clone EPR3861 )
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401727) , 20ug
100ul $325 In Stock
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WB(1)
IHC(1)
FC(1)
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Also for TMPRSS2 (NM_005656)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human TMPRSS2 was used as an immunogen.
Clone NameEPR3861 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, FC Suggested DilutionsFC: 1:100, ICC: 1:250 - 1,000, IHC: 1:1,000, WB: 1:1,000 - 10,000,
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens transmembrane protease, serine 2 (TMPRSS2), transcript variant 2
Alternative NamePP9284; PRSS10
Database LinkNP_005647
FunctionTransmembrane protease, serine 2 (TMPRSS2) is a type-II transmembrane serine protease. It encodes a type II trans-membrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. The biological function of TMPRSS2 is unknown; however, seine proteases are known to be involved in many physiological and pathological processes (1). TMPRSS2 is expressed highly in primary and metastatic prostate cancers. It is also regulated by androgens and is associated with tumor cell differentiation (1, 2), making it a potential target for cancer therapy and diagnosis. TMPRSS2-ERG fusion, which occurs on account of translocataions and interstitial deletions is implicated in aggressive forms of prostate cancer (3, 4).
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - TMPRSS2 antibody [EPR3861]; All lanes : Anti-TMPRSS2 antibody [EPR3861] at 1/1000 dilution.Lane 1 : Human prostate lysate.Lane 2 : Human small intestine lysate.Lane 3 : LNCaP cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/1000 dilution.Predicted band size : 54 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TMPRSS2 antibody [EPR3861]; TA307198 at 1/1000 dilution, staining TMPRSS2 in paraffin embedded Human prostatic adenocarcinoma
FC Image
Flow Cytometry - Anti-TMPRSS2 antibody [EPR3861]; Overlay histogram showing LoVo cells stained with TA307198 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

 

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