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Anti-TMPRSS2 Antibody EPR3861
Also for TMPRSS2 (NM_005656)
|A synthetic peptide corresponding to residues in human TMPRSS2 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||FC: 1:100, ICC: 1:250 - 1,000, IHC: 1:1,000, WB: 1:1,000 - 10,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens transmembrane protease, serine 2 (TMPRSS2), transcript variant 2|
|Transmembrane protease, serine 2 (TMPRSS2) is a type-II transmembrane serine protease. It encodes a type II trans-membrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. The biological function of TMPRSS2 is unknown; however, seine proteases are known to be involved in many physiological and pathological processes (1). TMPRSS2 is expressed highly in primary and metastatic prostate cancers. It is also regulated by androgens and is associated with tumor cell differentiation (1, 2), making it a potential target for cancer therapy and diagnosis. TMPRSS2-ERG fusion, which occurs on account of translocataions and interstitial deletions is implicated in aggressive forms of prostate cancer (3, 4). |
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Western blot - TMPRSS2 antibody [EPR3861]; All lanes : Anti-TMPRSS2 antibody [EPR3861] at 1/1000 dilution.Lane 1 : Human prostate lysate.Lane 2 : Human small intestine lysate.Lane 3 : LNCaP cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/1000 dilution.Predicted band size : 54 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TMPRSS2 antibody [EPR3861]; ab92323 at 1/1000 dilution, staining TMPRSS2 in paraffin embedded Human prostatic adenocarcinoma
Flow Cytometry - Anti-TMPRSS2 antibody [EPR3861]; Overlay histogram showing LoVo cells stained with ab92323 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.