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Home Antibody All anti-TK1 antibodies

Anti-TK1 Antibody EPR3193

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303718
  • Rabbit monoclonal antibody against Thymidine Kinase 1 (EPR3193 )
  • FREE positive control: HEK293T cell transient overexpression lysate (LC418805) , 20ug
100ul $325 3-7 Days
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WB(1)
IHC(1)
FC(1)
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Also for TK1 (NM_003258)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human Thymidine Kinase 1 was used as an immunogen.
Clone NameEPR3193 IsotypeIgG
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, FC Suggested DilutionsICC/IF: 1:100; WB: 1:5000 - 1:50000; IP: 1:10; IHC-P: 1:100 - 1:250; FC: 1:70
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens thymidine kinase 1, soluble (TK1)
Alternative NameTK2
Database LinkNP_003249
Function
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Thymidine Kinase 1 antibody [EPR3193]; Anti-Thymidine Kinase 1 [EPR3193] antibody at 1/50000 dilution + Molt-4 cell lysate at 10 µg.Secondary.HRP labelled goat anti-rabbit at 1/1000 dilution.Predicted band size : 25 kDa.Observed band size : 25 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Thymidine Kinase 1 antibody [EPR3193]; Immunohistochemical analysis of paraffin-embedded human tonsils using 1/100 TA303718
FC Image
Flow Cytometry-Anti-Thymidine Kinase 1 antibody [EPR3193](TA303718); Overlay histogram showing HeLa cells stained with TA303718 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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