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Home Antibody All anti-TGFB1 antibodies

Anti-TGFB1 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319281
  • Rabbit polyclonal TGF beta 1 antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC424586) , 20ug Explanation
100ug 325 3-7 Days
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WB(1)
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Also for TGFB1 (NM_000660)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenTGF beta 1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of the mature growth factor (112 amino acids in length).
Clone Name IsotypeIgG
Species Reactivityhuman Concentration0.77 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:40,000, WB: 1:100 - 1:500, IF: User Optimized
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note TGF-beta-1 is a multifunctional cytokine that belongs to a superfamily of structurally related regulatory proteins, including three mammalian TGF-beta isoforms (TGF-beta-1, -beta-2, and -beta-3), activin/inhibins and bone morphogenetic proteins. The most abundant isoform, TGF-beta-1, is a 25kDa homodimer composed of two 12.5 kDa subunits joined by disulfide bonds. TGF-beta-1 is a highly conserved molecule; the amino acid sequence between human and mouse differs by only one residue. Although originally defined by its ability to cause anchorage independent cell growth and changes in cell morphology of rat fibroblasts, subsequent research has revealed that TGF-beta is actually a major growth inhibitor for most cell types. It is produced by a wide variety of cell and tissue types during all stages of cell differentiation. TGF-beta-1 sources include platelets, bone and soft tissues, such as placenta and kidneys.

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis using Affinity Purified anti-TGF beta 1 antibody to detect human TGF beta 1. Each lane contains 250 ng of protein under non-reducing (lane 1) and reducing conditions (lane 2). Comparison to molecular weight markers (not shown) was used to estimate the indicated molecular weights. The blot was incubated with a 1:200 dilution of the antibody at room temperature for 1 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG (H&L) (611-132-122) diluted 1:2,500. IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

 

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