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Anti-TERT Antibody Y182
Also for TERT (NM_198253)
|A synthetic peptide corresponding to residues in the C-terminus of human TERT was used as immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000; ICC/IF: 1:50 - 1:100; FC: 1:100; IP: Use at an assay dependent dilution; IHC-P: Use at an assay dependent dilution; IHC-Fr: Use a concentration of 0.75 ug/ml
|Does not react with Mouse, Rat. Is unsuitable for ICC.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for ICC.
|Homo sapiens telomerase reverse transcriptase (TERT), transcript variant 1|
|CMM9; DKCA2; DKCB4; EST2; hEST2; hTRT; PFBMFT1; TCS1; TP2; TRT|
|Human telomerase reverse transcriptase (hTERT) is the catalytic subunit of the telomerase (1-2). It is also responsible for addition of TTAGGG repeats to the telomere ends of the chromosome, which is important in maintaining cell immortality. (3). In most normal human cells the expression of hTERT is very low. On the other hand, greater than 90 % of human tumors of different pathology and origins have been shown to upregulate telomerase expression (4), making hTERT a cancer-associated target |
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Western blot - Telomerase antibody [Y182]; Anti-Telomerase reverse transcriptase antibody [Y182] at 1/1000 dilution + Hela cell lysate.Predicted band size : 127 kDa.Observed band size : 122 kDa .
Immunofluorescence - Telomerase antibody [Y182]; Ab32020, at a 1/50 dilution, staining Telomerase Reverse Transcriptase in HeLa cells by Immunofluorescence.
Immunocytochemistry/ Immunofluorescence - Telomerase antibody [Y182]; ab32020 stainingTelomerase inhuman breast carcinoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.5%Triton x100and blocking with 2% serumwas performed for 30 minutes in 370C. Samples were incubated with primary antibody (1/100) in PBS for 1 hour30 minutes at 37°C. An Alexa Fluor? 594-conjugatedgoat monoclonal torabbit IgG was used at dilution at1/75 as secondary antibody.