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Anti-FEN1 TRUEMAB Antibody Clone OTI1F3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA802762 FEN1 mouse monoclonal antibody, clone OTI1F3 (formerly 1F3) 100ul $325 In Stock
LC401328 FEN1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF802762 Carrier-free (BSA/glycerol-free) FEN1 mouse monoclonal antibody, clone OTI1F3 (formerly 1F3) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(2)
IHC(9)

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 117-380 of human FEN1 (NP_004102) produced in E.coli.
Clone NameClone OTI1F3 IsotypeIgG1
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC Suggested Dilutions WB 1:2000, IHC 1:150,
Predicted MW Explanation 42.4 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens flap structure-specific endonuclease 1 (FEN1)
Alternative NameFEN-1; MF1; RAD2
Database LinkNP_004102
Entrez Gene 2237 Human
FunctionThe protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq, Jul 2008].
Related PathwayStem cell - PluripotencyDruggable Genome DNA replicationBase excision repairNon-homologous end-joining

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FEN1 (RC201785, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FEN1.
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Western blot analysis of  A549 cell lysate (35ug) by using anti-FEN1 monoclonal antibody. Dilution: 1:500
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-FEN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802762)
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-FEN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802762)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-FEN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802762)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FEN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802762)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FEN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802762)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-FEN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802762)
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FEN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802762)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FEN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802762)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-FEN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802762)
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