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Home Antibody All anti-DOT1L antibodies

Anti-DOT1L TRUEMAB Antibody Clone OTI1F1D4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA802742 DOT1L mouse monoclonal antibody, clone OTI1F1D4 (formerly 1F1D4) 100ul $325 In Stock
LC410109 DOT1L HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF802742 Carrier-free (BSA/glycerol-free) DOT1L mouse monoclonal antibody, clone OTI1F1D4 (formerly 1F1D4) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(1)
IHC(1)

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 1008-1424 of human DOT1L (NP_115871) produced in E.coli.
Clone NameClone OTI1F1D4 IsotypeIgG1
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC Suggested Dilutions WB 1:2000, IHC 1:150,
Predicted MW Explanation 164.7 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens DOT1-like histone H3K79 methyltransferase (DOT1L)
Alternative NameDOT1; KMT4
Database LinkNP_115871
Entrez Gene 84444 Human
FunctionThe protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011].
Related PathwayDruggable Genome Lysine degradation

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L (RC218547, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOT1L.
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DOT1L mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA802742)
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