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Home Antibody All anti-KIT antibodies

Anti-KIT GAT Antibody Polyclonal

Generated by Genomic Immunization


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA590744 Rabbit Polyclonal c-Kit Antibody 100ug $325 In Stock
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Also for KIT (NM_001093772)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the N Terminus Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman Concentration0.80511 mg/ml
Guaranteed Application *WB, ELISA, IHC Suggested Dilutions WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog (KIT), transcript variant 2
Alternative NameC-Kit; CD117; PBT; SCFR
Database LinkNP_001087241
Entrez Gene 3815 Human
Functionc-Kit is a transmembrane tyrosine kinase encoded by the cKit proto oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Mutations in c-Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer. Phosphorylation at tyrosine 721 of c-Kit allows binding and activation of PI3 kinase.
Related PathwayProtein KinaseES Cell Differentiation/IPSTransmembraneStem cell - PluripotencyAdult stem cellsEmbryonic stem cellsDruggable Genome Cytokine-cytokine receptor interactionEndocytosisHematopoietic cell lineageMelanogenesisPathways in cancerAcute myeloid leukemiaMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KIT (RC213772, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KIT.

WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KIT polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

ELISA: c-Kit Antibody

IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-KIT rabbit polyclonal antibody. (TA590744)


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