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Home Antibody All anti-GRN antibodies
UltraMAB

Anti-GRN GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA590710
  • Rabbit Polyclonal Granulin Antibody
50ug 325 In Stock
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WB(3)
IHC(1)
IF(1)
ELISA(1)
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Also for GRN (NM_002087)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the Middle Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman , Dog , Monkey , Mouse Concentration1mg/ml
Guaranteed Application *WB, ELISA, IHC, IF Suggested Dilutions WB 1:5000~20000,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens granulin (GRN)
Alternative NameCLN11; GEP; GP88; PCDGF; PEPI; PGRN
Database LinkNP_002078
FunctionGranulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.
Related Pathway

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WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRN (RC202139, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRN.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GRN polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
WB Image
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
ELISA Image
ELISA: Granulin Antibody - Affinity Purified
IHC Image
Immunohistochemical staining of human epididymis shows strong cytoplasmic positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
IF Image
Immunofluorescent staining of human cell line U-2 OS shows no positivity.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.

 

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