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Anti-AURKB GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for AURKB (NM_004217)
|DNA immunization. This antibody is specific for the N Terminus Region of the target protein.|
|Human , Dog , Monkey , Mouse
|WB, ELISA, IHC
|| WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens aurora kinase B (AURKB), transcript variant 1|
|AIK2; AIM-1; AIM1; ARK2; AurB; aurkb-sv1; aurkb-sv2; IPL1; PPP1R48; STK12; STK5|
|AURKB (aurora kinase B, AIK2 or aurora-B), with 344-amino acid protein(about 39kDa),localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. AURKB is a mitotic protein kinase, which phosphorylates histone H3 and regulates Chromosomal segregation during mitosis and meiosis. It may regulates several stages of mitosis such as centrosome separation, chromosome segregation and cytokinesis. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. ARK-2 transcripts arepresent at high levels in human thymus and fetal liver. ARK-2 protein levels are maximal during both S and G2/M phases.|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AURKB (RC210288, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AURKB.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AURKB polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-AURKB rabbit polyclonal antibody. (TA590640)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-AURKB rabbit polyclonal antibody. (TA590640)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-AURKB rabbit polyclonal antibody. (TA590640)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-AURKB rabbit polyclonal antibody. (TA590640)