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Anti-ELK1 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for ELK1 (NM_005229)
|DNA immunization. This antibody is specific for the C Terminus Region of the target protein.|
|WB, ELISA, IHC
||WB 1:20000, IHC 1:150, ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens ELK1, member of ETS oncogene family (ELK1), transcript variant 2|
|ELK1 protein; ETS-like gene 1; OTTHUMP00000023224; OTTHUMP00000023225; ELK1, member of ETS oncogene family|
|The transcription factor ELK1 is a family of member of ETS oncogene family and of the ternary complex factor (TCF) subfamily,which is located on chromosome Xp11.2 and stimulates transcription. binds to purine-rich DNA sequences. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C terminus,It appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo. More recent studies have shown that Elk1 is also a target of the Stress Activated Kinase SAPK/JNK. Phosphorylation of Elk1 has also been implicated in synaptic plasticity in the adult hippocampus.|
EGFR1 Signaling Pathway
MAPK signaling pathway
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ELK1 (RC208921, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ELK1.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-ELK1 rabbit polyclonal antibody. (TA590610)
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-ELK1 rabbit polyclonal antibody. (TA590610)