Home Antibody All anti-POU2AF1 antibodies
Anti-POU2AF1 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for POU2AF1 (NM_006235)
|DNA immunization. This antibody is specific for the C Terminus Region of the target protein.|
|| WB 1:5000~20000,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens POU class 2 associating factor 1 (POU2AF1)|
|BOB1; OBF-1; OBF1; OCAB|
|A novel 35kDa protein designated Bob1 (B cell Oct binding protein 1) is a lymphocyte specific transcription co activator protein. B cell specific transcription is mediated by octamer motifs found in immunoglobulin heavy and light chain gene promoters and in some immunoglobulin enhancers. These sites are bound by the B cell specific Oct1 and Oct2 transcription factors, and the B cell restricted co activator BOB.1/OBF.1. This protein interacts only with the Oct1/2 proteins through sub domains in the POU domain of the Oct1/2 proteins, enhancing their transcriptional efficacy. Although having no intrinsic capacity for DNA binding, Bob1 associates tightly with the octomer motif in the presence of Oct1 and Oct2. Bob1 is expressed at highest levels in spleen and peripheral blood leukocytes.|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU2AF1 (RC207562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU2AF1.