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Home Antibody All anti-HMMR antibodies
UltraMAB

Anti-HMMR GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA590241 Rabbit Polyclonal CD168 Antibody 50ug $325 In Stock
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WB(2)
ELISA(1)

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the Middle Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman Concentration1.01mg/ml
Guaranteed Application *WB, ELISA Suggested Dilutions WB 1:20000,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens hyaluronan-mediated motility receptor (RHAMM) (HMMR), transcript variant 2
Alternative NameCD168; IHABP; RHAMM
Database LinkNP_036616
Entrez Gene 3161 Human
FunctionCD168 is involved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including the focal adhesion kinase occurs. CD168 may also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity.
Related PathwayStem cell - PluripotencyDruggable Genome ECM-receptor interaction

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HMMR (RC213321, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HMMR.
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Western Blot: CD168 Antibody - Detection of HMMR in 293T cell extract using anti-human HMMR antibody (3500.00.02) at a dilution of 1:500 with a stock of 1 mg/mL and with donkey anti-rabbit HRP secondary antibody at a dilution of 1:10,000. Lane one (-): 293T cell extract. Lane two (+): 293T cells transfected with full length RHAMM. Cells were lysed with NP40 lysis buffer. 20ug proteins were loaded per lane. Depticted molecular weight of human HMMR is 84.1 kDa. Image courtesy of Yi-Chieh Nancy Du, Memorial Sloan-Kettering Cancer Center.
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ELISA: CD168 Antibody
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