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Anti-HMMR GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for HMMR (NM_012484)
|DNA immunization. This antibody is specific for the Middle Region of the target protein.|
|| WB 1:20000,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens hyaluronan-mediated motility receptor (RHAMM) (HMMR), transcript variant 2|
|CD168; IHABP; RHAMM|
|CD168 is involved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including the focal adhesion kinase occurs. CD168 may also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity.|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HMMR (RC213321, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HMMR.
Western Blot: CD168 Antibody - Detection of HMMR in 293T cell extract using anti-human HMMR antibody (3500.00.02) at a dilution of 1:500 with a stock of 1 mg/mL and with donkey anti-rabbit HRP secondary antibody at a dilution of 1:10,000. Lane one (-): 293T cell extract. Lane two (+): 293T cells transfected with full length RHAMM. Cells were lysed with NP40 lysis buffer. 20ug proteins were loaded per lane. Depticted molecular weight of human HMMR is 84.1 kDa. Image courtesy of Yi-Chieh Nancy Du, Memorial Sloan-Kettering Cancer Center.