Home Antibody All anti-BTN3A2 antibodies
Anti-BTN3A2 GAT Antibody Polyclonal
Generated by Genomic Immunization
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Also for BTN3A2 (NM_007047)
|DNA immunization. This antibody is specific for the C Terminus Region of the target protein.|
|Human , Dog , Mouse
|WB, ELISA, IHC, IF
|| WB 1:5000~20000, IHC 1:150, ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens butyrophilin subfamily 3 member A2 (BTN3A2), transcript variant 1|
|BT3.2; BTF4; BTN3.2; CD277|
Entrez Gene 11118 Human
Entrez Gene 481727 Dog
|This gene encodes a member of the immunoglobulin superfamily, containing two Ig domains with similarity to Ig variable and Ig constant domains. The gene resides in the juxta-telomeric region of the major histocompatability class 1 locus on chromosome 6 in the seven member BTN cluster, which includes butyrophilin, and three members each of the BTN2 and BTN3 subfamilies. Alternatively spliced transcript variants have been described but their full-length nature has yet to be determined.|
|TransmembraneDruggable Genome |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTN3A2 (RC201183, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN3A2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BTN3A2 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BTN3A2 rabbit polyclonal antibody. (TA590233)
Immunohistochemistry: BTN3A2 Antibody - Most of the normal tissues displayed moderate cytoplasmic positivity with an additional membranous staining in gastro-intestinal tract. Smooth muscle cells and lymphoid cells outside reaction centra showed strong cytoplasmic staining while strong nuclear staining was seen in a fraction of cells in seminiferous duct. Liver, renal tubules, prostate glands and neuronal tissues were weakly stained or negative. Malignant tissues exhibited similar staining properties as normal cells, mainly moderate cytoplasmic and/or membranous immunoreactivity. Many gliomas, prostate, skin, testicular, urothelial and renal cancers were weakly stained or negative. Image and statement courtesy of the Human Protein Atlas (HPA).
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunofluorescent staining of human cell line U-2 OS shows no positivity.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.