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Home Antibody All anti-VAPB antibodies
UltraMAB

Anti-VAPB GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA590191
  • Rabbit Polyclonal VAPB Antibody
100ug 325 In Stock
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WB(3)
IHC(5)
IF(1)
ELISA(1)

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the C Terminus Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration0.9mg/ml
Guaranteed Application *WB, ELISA, IHC, IF Suggested Dilutions WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens VAMP (vesicle-associated membrane protein)-associated protein B and C (VAPB), transcript variant 1
Alternative NameALS8; VAMP-B; VAP-B
Database LinkNP_004729
Entrez Gene 9217 Human
Entrez Gene 56491 Mouse
Entrez Gene 60431 Rat
Entrez Gene 703156 Monkey
Entrez Gene 477274 Dog
FunctionThe protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking. [provided by RefSeq]
Related PathwayTransmembrane

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VAPB (RC200517, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VAPB.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-VAPB polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
WB Image
Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
ELISA Image
ELISA: VAPB Antibody
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-VAPB Rabbit Polyclonal antibody. (TA590191)
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-VAPB Rabbit Polyclonal antibody. (TA590191)
IHC Image
Immunohistochemistry: VAPB Antibody - Immunohistochemical staining for vesicle-associated membrane protein B/C (VAPB) in human uterine corpus. Glandular cells stained strongly while cells in the endometrial stroma did not stain or stained weakly. This image is the courtesy of the Human Proteome Resource (HPR).
IHC Image
Immunohistochemistry: VAPB Antibody - Normal tissues generally showed strong cytoplasmic staining usually with membranous positivity. Malignant tissues displayed similar staining pattern as their normal counterparts. Image and statement courtesy of the Human Protein Atlas (HPA).
IHC Image
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
IF Image
Immunofluorescent staining of human cell line U-2 OS shows positivity in endoplasmic reticulum & golgi apparatus.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.

 

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