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Home Antibody All anti-GCLM antibodies
UltraMAB

Anti-GCLM GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA590111 Rabbit Polyclonal GCLM Antibody 100ug $325 In Stock
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WB(2)
ELISA(1)

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the N Terminus Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman , Rat , Monkey , Mouse Concentration1.21898 mg/ml
Guaranteed Application *WB, ELISA Suggested Dilutions WB 1:5000~20000, ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens glutamate-cysteine ligase, modifier subunit (GCLM)
Alternative NameGLCLR
Database LinkNP_002052
Entrez Gene 2730 Human
Entrez Gene 14630 Mouse
Entrez Gene 29739 Rat
FunctionGlutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.
Related PathwayDruggable Genome Glutathione metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GCLM (RC207224, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GCLM.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GCLM polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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ELISA: GCLM Antibody - Affinity Purified
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