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Home Antibody All anti-AFP antibodies
UltraMAB

Anti-AFP GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA590065
  • Rabbit Polyclonal Alpha 1 Fetoprotein Antibody
100ug 325 In Stock
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WB(2)
IHC(15)
ELISA(1)
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Also for AFP (NM_001134)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the Middle Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration1.2mg/ml
Guaranteed Application *WB, ELISA, IHC Suggested Dilutions WB 1:5000~20000, IHC 1:150,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens alpha-fetoprotein (AFP)
Alternative NameFETA; HPAFP
Database LinkNP_001125
Entrez Gene 174 Human
Entrez Gene 11576 Mouse
Entrez Gene 24177 Rat
Entrez Gene 704786 Monkey
Entrez Gene 403551 Dog
FunctionAlpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFP (RC206622, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFP.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AFP polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
ELISA Image
ELISA: Alpha 1 Fetoprotein Antibody
IHC Image
Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-AFP rabbit polyclonal antibody. (TA590065)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-AFP rabbit polyclonal antibody. (TA590065)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-AFP rabbit polyclonal antibody. (TA590065)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-AFP rabbit polyclonal antibody. (TA590065)
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-AFP rabbit polyclonal antibody. (TA590065)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-AFP rabbit polyclonal antibody. (TA590065)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-AFP rabbit polyclonal antibody. (TA590065)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-AFP rabbit polyclonal antibody. (TA590065)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-AFP rabbit polyclonal antibody. (TA590065)
IHC Image
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-AFP rabbit polyclonal antibody. (TA590065)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AFP rabbit polyclonal antibody. (TA590065)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-AFP rabbit polyclonal antibody. (TA590065)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-AFP rabbit polyclonal antibody. (TA590065)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AFP rabbit polyclonal antibody. (TA590065)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-AFP rabbit polyclonal antibody. (TA590065)

 

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