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Anti-RAPGEF1 TRUEMAB Antibody Clone 3H5
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for RAPGEF1 (NM_198679)
|Full length human recombinant protein of human RAPGEF1(NP_941372) produced in HEK293T cell.|
|Human , Monkey
|| WB 1:400~4000, IF 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 2|
|This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAPGEF1 (RC208092, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAPGEF1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RAPGEF1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunofluorescent staining of HeLa cells using anti-RAPGEF1 mouse monoclonal antibody (TA506854).