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Home Antibody All anti-RAPGEF1 antibodies

Anti-RAPGEF1 TRUEMAB Antibody Clone 3H5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA506854
  • RAPGEF1 mouse monoclonal antibody,clone 3H5
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC404802) , 20ug Explanation
100ul 325 In Stock
CF506854
  • Carrier-free (BSA/glycerol-free) RAPGEF1 mouse monoclonal antibody,clone 3H5
100ug $450 8-10 Days
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WB(2)
IF(1)
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Also for RAPGEF1 (NM_198679)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human RAPGEF1(NP_941372) produced in HEK293T cell.
Clone NameClone 3H5 IsotypeIgG2b
Species ReactivityHuman , Monkey Concentration1 mg/ml
Guaranteed Application *WB, IF Suggested Dilutions WB 1:400~4000, IF 1:100,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 2
Alternative NameC3G; GRF2
Database LinkNP_941372
Entrez Gene 2889 Human
Entrez Gene 715272 Monkey
FunctionThis gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008].
Related PathwayDruggable Genome Focal adhesionNeurotrophin signaling pathwayInsulin signaling pathwayRenal cell carcinoma

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAPGEF1 (RC208092, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAPGEF1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RAPGEF1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
IF Image
Immunofluorescent staining of HeLa cells using anti-RAPGEF1 mouse monoclonal antibody (TA506854).

 

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