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Home Antibody All anti-POU5F1 antibodies

Anti-POU5F1 TRUEMAB Antibody Clone 7H1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA506798
  • POU5F1 mouse monoclonal antibody,clone 7H1
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400950) , 20ug
100ul 325 In Stock
CF506798
  • Carrier-free (BSA/glycerol-free) POU5F1 mouse monoclonal antibody,clone 7H1
100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(2)
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Also for POU5F1 (NM_002701)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human POU5F1(NP_002692) produced in HEK293T cell.
Clone NameClone 7H1 IsotypeIgG1
Species ReactivityHuman , Rat , Mouse Concentration1.00mg/ml
Guaranteed Application *WB Suggested Dilutions WB 1:200~1000,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens POU class 5 homeobox 1 (POU5F1), transcript variant 1
Alternative NameOct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4
Database LinkNP_002692
FunctionThis gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Mar 2010].
Related Pathway
Wnt Signaling Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU5F1 (RC211998, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU5F1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-POU5F1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

 

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