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Anti-BID TRUEMAB Antibody Clone OTI3E9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA506766 BID mouse monoclonal antibody, clone OTI3E9 (formerly 3E9) 100ul $325 In Stock
LC420074 BID HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF506766 Carrier-free (BSA/glycerol-free) BID mouse monoclonal antibody, clone OTI3E9 (formerly 3E9) 100ug $450 8-10 Days
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WB(2)
IHC(3)
IF(2)

OriGene Data

ImmunogenFull length human recombinant protein of human BID(NP_001187) produced in HEK293T cell.
Clone NameClone OTI3E9 IsotypeIgG2a
Species ReactivityHuman , Monkey , Mouse Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF Suggested Dilutions WB 1:400~4000, IHC 1:150, IF 1:100,
Predicted MW Explanation 21.8 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens BH3 interacting domain death agonist (BID), transcript variant 2
Alternative NameFP497
Database LinkNP_001187
Entrez Gene 637 Human
Entrez Gene 12122 Mouse
Function
Related PathwayDruggable Genome p53 signaling pathwayApoptosisNatural killer cell mediated cytotoxicityAlzheimer's diseaseAmyotrophic lateral sclerosis (ALS)Pathways in cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BID (RC207261, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BID.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BID monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-BID mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506766)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BID mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506766)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BID mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506766)
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Anti-BID mouse monoclonal antibody (TA506766) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BID(RC207261).
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Immunofluorescent staining of HeLa cells using anti-BID mouse monoclonal antibody (TA506766).
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