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Home Antibody All anti-EPCAM antibodies

Anti-EPCAM TRUEMAB Antibody Clone OTI1C1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA506629 EPCAM mouse monoclonal antibody,clone OTI1C1 100ul $325 In Stock
LC400847 EPCAM HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF506629 Carrier-free (BSA/glycerol-free) EPCAM mouse monoclonal antibody,clone OTI1C1 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(3)
IHC(2)
IF(1)
FC(3)
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Also for EPCAM (NM_002354)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human EPCAM(NP_002345) produced in HEK293T cell.
Clone NameClone OTI1C1 IsotypeIgG2b
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:400-1:1000, IHC: 1:150-1:500, IF: 1:100, Flow: 1:100
Predicted MW Explanation 32.7 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens epithelial cell adhesion molecule (EPCAM)
Alternative NameDIAR5; EGP-2; EGP314; EGP40; ESA; HNPCC8; KS1/4; KSA; M4S1; MIC18; MK-1; TACSTD1; TROP1
Database LinkNP_002345
Entrez Gene 4072 Human
FunctionThis gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008].
Related PathwayES Cell Differentiation/IPSTransmembrane

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPCAM (RC201989, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPCAM.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EPCAM monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)(1:400).
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Western blot analysis of extracts (10ug) from 1 cell line by using anti-EPCAM monoclonal antibody at 1:200.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-EPCAM mouse monoclonal antibody at 1:500 dilution. (TA506629)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-EPCAM mouse monoclonal antibody at 1:500 dilution. (TA506629)
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Anti-EPCAM mouse monoclonal antibody (TA506629) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EPCAM(RC201989).
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Flow cytometric analysis of live HEK293 cells transfected with EPCAM ORF clone (Cat# RC201989), using anti-EPCAM antibody (TA506629 at 1:100, Red), compared to IgG1 isotype control (Black).
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Flow cytometric analysis of live HT29 cells, using anti-EPCAM antibody (TA506629 at 1:100, Red), compared to IgG1 isotype control (Black).
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Flow cytometric analysis of live OVCAR-4 cells, using anti-EPCAM antibody (TA506629 at 1:100, Red), compared to IgG1 isotype control (Black).
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