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Anti-CD19 TRUEMAB Antibody Clone OTI3B10

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA506236 CD19 mouse monoclonal antibody, clone OTI3B10 (discontinued and upgraded to UltraMAB UM500071) 100ul $325 In Stock
CF506236 Carrier-free (BSA/glycerol-free) CD19 mouse monoclonal antibody, clone OTI3B10 (formerly 3B10) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(1)
IHC(4)
IF(1)
FC(3)
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OriGene Data

ImmunogenFull length human recombinant protein of human CD19(NP_001761) produced in HEK293T cell.
Clone NameClone OTI3B10 IsotypeIgG1
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested Dilutions WB 1:4000, IHC 1:150, IF 1:100, FLOW 1:50,
Predicted MW Explanation 60.9 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens CD19 molecule (CD19), transcript variant 2
Alternative NameB4; CVID3
Database LinkNP_001761
Entrez Gene 930 Human
FunctionLymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008].
Related PathwayTransmembraneDruggable Genome Hematopoietic cell lineageB cell receptor signaling pathwayPrimary immunodeficiency

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.


WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD19 (RC202922, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD19.
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IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD19 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, TA506236)(1:600)
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IHC Image
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD19 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min, TA506236)(1:600)
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IHC Image
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD19 mouse monoclonal antibody. (TA506236; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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IHC Image
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD19 mouse monoclonal antibody. (TA506236; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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IF Image
Anti-CD19 mouse monoclonal antibody (TA506236) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD19(RC202922).
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FC Image
Living HEK293T cells transfected with either CD19 (RC202922) overexpression plasmid or empty vector control plasmid were immunostained by either anti-CD19 antibody (TA506236) or a nonspecific negative control antibody (TA180143) and then analyzed by flow cytometry. CD19 (red) or empty vector(blue) transfected cells with anti-CD19 antibody. CD19 (green) or empty vector(aqua) transfected cells with control antibody.(1:100)
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FC Image
Flow cytometric Analysis of living RBC-lysed human peripheral blood cells, using anti-huamn CD19 antibody(TA506236),(Red), compared to a nonspecific negative control antibody(TA180144),(green) and PBS,(blue).(1:20)
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FC Image
Flow cytometric Analysis of living Raji cells, using anti-CD19 antibody(TA506236),(Red), compared to a nonspecific negative control antibody TA180143,(green), or PBS (blue).(1:20)
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